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Company can supply Avian Influenza Virus Test,

Supplied by Felco International Supplies, Inc.
1413 Larkwood Dr, Dewitt, MI 48820, USA

One step avian influenza virus antigen test for HPAI
AI Check H5 AIV Ag Test

■ Principles
AI Check H5 Avian Influenza Virus Antigen Test is a chromatographic immunoassay for the qualitative, differential detection of avian influenza type A subtype H5 virus antigen (High pathogenic avian influenza virus: HPAI) in avian feces.
AI Check H5 Avian Influenza Virus Antigen Test has a letter of T as test line (avian influenza subtype H5 virus) and C as control line on the surface of the device. Both the test line and control line in result window are not visible before applying any samples. The control line is used for procedural control. Control line should be always appeared if the test procedure is performed properly and the test reagents of control line are working. A purple test line respectively will be visible in the result window if there is enough avian influenza virus subtype H5 antigen in the specimen.
A monoclonal anti-avian influenza subtype Hemagglutinin 5 (H5) virus is used in test line as both capture and detector materials. These enable H5 Avian Influenza Virus Antigen Test to identify high pathogenic avian influenza subtype H5 virus antigen in avian feces tissues with a high degree of accuracy.

■ Materials provided (20 tests/kit)
1) AI Check H5 Avian Influenza Virus Antigen Test Devices x20
2) Specimen collection tubes containing 1ml of assay diluent x20
3) Sample collection swabs x20
4) Disposable droppers x20
5) Instruction for use

■ Precautions
1) For veterinary research use only.
2) For best results, strict adherence to there instructions is required.
3) All specimens should be handled as being potentially infectious.
4) Do not open or remove test kit from their individually sealed pouches until immediately before their use.
5) Do not use the test kit if the pouch is damaged or the seal is broken.
6) Do not reuse test kit.
7) All reagents must be at room temperature before running the assay.
8) Do not use reagents beyond the stated expiration date marked on the package label.
9) The components in this kit have been quality control tested as standard batch unit.
10) Do not mix components from different lot numbers.

■ Storage and Stability
The kit can be stored at room temperature (2~30℃) or refrigerated. The test kit is stable through the expiration date marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

■ Specimen Collection and Preparation [Refer to the Figure of detailed specimen collection & test procedure]
1) Avian feces tissue samples from cloaca swab of rectum should be used with this test.
2) If feces specimens are not immediately tested, they should be refrigerated at 2~8℃. For storage not less than 48hours, freeze the specimen at -20℃ or below.

■ Procedure of the test [Refer to the Figure of detailed specimen collection & test procedure]
1) Take a portion of feces tissue from cloaca swab of rectum with the sample collection swab.
2) Insert the swab into the specimen tube containing assay diluent.
3) Mix the swab until the sample has been dissolved into the assay diluent.
4) Leave the test tube until the large particles have settled down to the bottom of the tube.
5) Remove the test device from the foil pouch, and places it on a flat and dry surface.
6) Using the disposable dropper provided, take the supernatant from extracted sample in the tube.
7) Add 4 ~ 5 drops into the sample hole with disposable dropper.
8) As the test begins to work, you will see purple color move across the result window in the center of the test device.
9) Interpret test results at 20 ~ 30 minutes.

■ Interpretation of the test
A color line will appear in the right section of the result window to show that the test is working properly. This line is the Control Line. The left section of the result window indicates the test results. If another color line appears in the left section of the result window, this line is the Test Line 1 of avian influenza subtype H5 virus or/and Test line 2 of common avian influenza virus

1) Avian influenza subtype H5 virus positive reaction
The presence of 2-color (T, C) lines indicate a positive result for avian influenza subtype H5 virus.

3) Negative reaction
The presence of only one line (C) within the result window indicates a negative result.

4) Invalid
The test is invalid if the C line does not appear. If this occurs, the test should be repeated using a new strip.

■ Limitations of the test
1) This kit can detect all avian influenza virus type A (subtype H5). Although AI Check H5 Avian Influenza Virus Antigen Test is very accurate in detecting avian influenza virus antigen, a low incidence of false results can be occurred.
2) Although test result is only common avian influenza virus positive reaction, you should not completely exclude the possibility of avian influenza subtype H5 virus positive reaction. Therefore, other clinically available tests are required if questionable results are obtained.
3) As with all diagnostic tests, a definitive clinical diagnosis should not be based on the results of a single test, but should only be made by the veterinarian after all clinical and laboratory findings have been evaluated.

■ Cross-reactivity test with other H subtype of Avian influenza virus type A
Test line (H5) of H5 Avian Influenza Virus Antigen Test showed no cross-reactivity with other H subtype (H1 ~ H4, H6 ~ H15) antigen of Avian influenza virus type A

■ Bibliography
(1) V.S. Ivanov, Z.K. Suvorova, L.D. Tchikin, A.T. Kozhich and V.T. Ivanov : Effective method for synthetic peptide immobilization that increases the sensitivity and specificity of ELISA procedures. Journal of Immunological Methods, 153 (1992) 229-233.
(2) Kodilhalli S, Sivandan V, Nagraja KV, Goyal SM, Halvorson DA ; Antigen capture enzyme immunoassay for detection of avian influenza virus in turkeys ; American Journal of Veterinary Research 1993 Sep:54(9) : 1385-90
(3) Marcante R, Chiumento F, Paul G, Cavedon G : Rapid diagnosis of influenza type A infection: comparison of shell-vial culture direct antigen flu-A and enzyme linked immunosorbent assay: New Microbiology/ 1996 Apr;19(2) 141-7
(4) Siebinga JT, de Boer GF.: Influenza A viral nucleoprotein detection in isolates from human and various animal species. Archives of Virology 1988;100(1-2):75-87
(5) Huaguang Lu. : A longitudinal study of a novel dot-enzyme-linked immunosorbent assay for detection of avian influenza virus : Avian Diseases: Vol. 47, No. s3, pp. 10151021.

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